Poly-(lactic acid) and fibrin bioactive cellularized scaffold for use in bone regenerative medicine: Proof of concept
| Autor: | Valmir Vieira Rocha Júnior, André Capaldo Amaral, Mônica Rosas da Costa Iemma, Heloisa Sobreiro Selistre de Araújo, Antonio J. F. Carvalho, Renata Aquino de Carvalho, Eliane Trovatti |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Scaffold Polymers and Plastics biology Mesenchymal stem cell Bioengineering 02 engineering and technology 021001 nanoscience & nanotechnology Regenerative medicine Fibrin Lactic acid Cell biology Biomaterials 03 medical and health sciences chemistry.chemical_compound chemistry Platelet-rich plasma Materials Chemistry biology.protein 0210 nano-technology 030304 developmental biology |
| Zdroj: | Journal of Bioactive and Compatible Polymers. 36:171-184 |
| ISSN: | 1530-8030 0883-9115 |
| Popis: | Bone regenerative medicine (BRM) aims to overcome the limitations of conventional treatments for critical bone defects by developing therapeutic strategies, based on temporary bioactive substitutes, capable of stimulating, sustaining, and guiding tissue regeneration. The aim of this study was to validate the “proof of concept” of a cellularized bioactive scaffold and establish its potential for use in BRM. For this purpose, three-dimensional scaffolds of poly-(lactic acid) (PLA), produced by the additive manufacturing technique, were incorporated into a human platelet-rich plasma (PRP-h) fibrin matrix containing human infrapatellar fat pad mesenchymal stem cells (hIFPMSC). The scaffolds (PLA/finbrin-bioactive) were kept under ideal culture conditions in a medium free from fetal bovine serum and analyzed at 5 and 10 days by Scanning Electron Microscopy (SEM), Fourrier Transform Infrared (FTIR), Circular Dichroism and fluorescence microscopy. The results demonstrated the feasibility of obtaining a rigid, cytocompatible, and cellularized three-dimensional structure. In addition, PRP platelets and leukocytes were able to provide a bioactive environment capable of maintaining the viability of hIFPMSC into scaffolds. The results validate the concept of a customizable, bioactive, cellularized, and non-immunogenic strategy for application in BRM. |
| Databáze: | OpenAIRE |
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