Are K+ channels and H+‐ATPases of the plasma membrane involved in the control and generation of circadian rhythmicity in pulvinar motor cells of phaseolus?
| Autor: | Sabine Schöffel, W. E. Mayer, Sharon Betz |
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| Rok vydání: | 1994 |
| Předmět: | |
| Zdroj: | Biological Rhythm Research. 25:301-314 |
| ISSN: | 1744-4179 0929-1016 |
| DOI: | 10.1080/09291019409360305 |
| Popis: | The circadian leaf movement of Phaseolus coccineus L. is mediated by ion fluxes across the plasma membrane of the pulvinar motor cells. H+‐ATPases and K+ channels of the plasma membrane are key molecules which produce the responses to external and internal signals by directing the flow of ionic osmotica for the swelling and shrinking of the pulvinar motor cells. We investigated the involvement of these molecules in the circadian rhythmicity of the Phaseolus pulvinus by testing the phase‐shifting effects and /or the period‐changing effects of inhibitors of the plasma membrane H+‐ATPase, of other treatments which reduce the H+ transport across the plasma membrane, and of the K+ channel blocker TEA on the leaf movement rhythm. Four hour pulses of vanadate (0.4 mM) and erythrosin B (8 pM), of low temperature (3–6 °C, down from an otherwise constant 23 °C) and of TEA (4 mM) shift the phase of the leaf movement rhythm. The phase response curves are similar in shape and resemble those for cyanide and az... |
| Databáze: | OpenAIRE |
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