A highly selective 'turn-on' fluorescent sensor for zinc ion based on a cinnamyl pyrazoline derivative and its imaging in live cells
| Autor: | Huichen Guo, Chun-Mei Ma, Xiao-Yu Liu, Ying-Peng Zhang, Jiaxi Ru, Yun-Shang Yang, Qing-Hua Xue |
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| Rok vydání: | 2018 |
| Předmět: |
inorganic chemicals
Detection limit Membrane permeability 010405 organic chemistry Chemistry General Chemical Engineering General Engineering Pyrazoline 010402 general chemistry 01 natural sciences Fluorescence 0104 chemical sciences Analytical Chemistry Ion Chemometrics chemistry.chemical_compound Titration Derivative (chemistry) Nuclear chemistry |
| Zdroj: | Analytical Methods. 10:1833-1841 |
| ISSN: | 1759-9679 1759-9660 |
| Popis: | A fluorescence probe based on cinnamyl pyrazoline was developed and synthesized. The probe detected Zn2+ ion that was mixed with a series of cations in aqueous ethanol with high selectivity and high sensitivity. After adding Zn2+, the fluorescence intensity was significantly enhanced, with a 56-fold increase. The detection limits and binding constants of 2.95 × 10−8 M and 1.468 × 104 M−1 were calculated by a fluorescence titration experiment. The 1 : 1 binding chemometrics of the 1–Zn2+ complex was analyzed by Job's plot. The probe showed good reversibility when adding PPi. Additionally, the “turn-on” fluorescent probe with excellent membrane permeability was satisfactorily applied to microscopic imaging for the detection of Zn2+ in baby hamster kidney (BHK)-21 cells. |
| Databáze: | OpenAIRE |
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