ImprovedIn VitroBiocompatibility of Bicarbonate-Buffered Peritoneal Dialysis Fluid
| Autor: | R. Taamma, Pierre Gane, Marie-Paule Wautier, Eric Boulanger, Nicolas Grossin, Jean-Luc Wautier |
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| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty business.industry Cell growth medicine.medical_treatment 030232 urology & nephrology General Medicine Peritoneal dialysis Andrology Vascular endothelial growth factor 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine L-Glucose chemistry Nephrology Apoptosis Glycation Medicine 030212 general & internal medicine business Peritoneal Fibrosis Mesothelial Cell |
| Zdroj: | Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis. 26:664-670 |
| ISSN: | 1718-4304 0896-8608 |
| Popis: | BackgroundConventional peritoneal dialysis fluids (PDFs) have been shown to damage the mesothelial layer and are associated with the development of peritoneal fibrosis and neoangiogenesis. New-generation PDFs have therefore been developed with physiological pH and reduced levels of glucose degradation products (GDPs), precursors of advanced glycation end products (AGEs). In this work, we evaluated and compared the improved biocompatibility of two new-generation PDFs (Balance and bicaVera) using mesothelial cell biology; we also compared them to a standard PDF (stay·safe) (all PDFs by Fresenius Medical Care, Fresnes, France).Methodsstay·safe, Balance, and bicaVera were tested for their effect on human peritoneal mesothelial cell (HPMC) viability by measuring cell proliferation and apoptosis, and oncosis induction. The formation of AGEs was evaluated by immunoassay. Transforming growth factor beta-1 and vascular endothelial growth factor (VEGF) were immunoassayed in HPMC supernatants exposed to the above PDFs.ResultsAt 15 g/L glucose concentration, HPMC exposure to bicaVera resulted in higher cell proliferation compared to Balance ( p < 0.001) and stay·safe ( p < 0.001). Compared to the lactate-buffered PDFs (Balance and stay·safe), oncosis was significantly lower in cells exposed to bicaVera ( p < 0.05). bicaVera, containing lower amounts of GDPs, generated less AGE formation ( p < 0.05) and VEGF production ( p < 0.05) than either Balance or stay·safe.ConclusionsNew-generation PDFs with physiological pH and lower GDP levels, especially if bicarbonate-buffered (bicaVera), have fewer in vitro toxic effects on mesothelial cells and may contribute to peritoneal preservation, thus improving long-term treatment of PD patients. |
| Databáze: | OpenAIRE |
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