Natural bioactive from Euphorbia umbellata latex as a modulator of mitochondrial activity in breast cancer cells

Autor:	Amanda Gomes, Thalles Anthony Duarte Oliveira, Thais de Oliveira Conceição, Durvanei Augusto Maria, Rosa Andrea Nogueira Laiso, Monique Gonçalves Alves, Laertty Garcia de Sousa Cabral
Rok vydání:	2020
Předmět:	chemistry.chemical_compound Chloroform Ethanol chemistry Biochemistry Apoptosis Confocal microscopy law Cytotoxic T cell Ether Cytotoxicity law.invention Dichloromethane
Zdroj:	Journal of Pharmacognosy and Phytochemistry. 9:823-829
ISSN:	2278-4136 2349-8234
Popis:	The objective of this study was to evaluate the antiproliferative effect, antitumor and modulation of the mitochondrial electrical potential of the acetate and chloroform fractions and the methanol, ethanol, dichloromethane and ether subfractions extracted from Euphorbia umbellata. The cytotoxicity of fractions and subfractions was evaluated by the MTT colorimetric assay after 24h at concentrations ranging from 2-30 μg/mL, also the mitochondrial electrical potential by confocal microscopy. A reduction in cell viability was observed, with cytotoxicity in most treatments for all cells with a marked reduction in mitochondrial electrical potential. Possibly the treatments are modulating regulated cell death pathways. The acetate and chloroform fractions showed the highest cytotoxic potential with greater selectivity for tumor cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fabc12a790b4196169067a499948b15a https://doi.org/10.22271/phyto.2020.v9.i5l.12326 Zobrazit plný text záznamu