Thin-layer chromatography–contact bioautography as a tool for bioassay-guided isolation of anti-Streptococcus mutanscompounds fromPinus merkusiiheartwood
| Autor: | Apirak Sakunpak, Lukman Sueree |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chromatography biology 030106 microbiology 010401 analytical chemistry Clinical Biochemistry Broth microdilution Ethyl acetate biology.organism_classification 01 natural sciences Biochemistry Streptococcus mutans High-performance liquid chromatography Thin-layer chromatography 0104 chemical sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound chemistry Isopimaric acid Abietic acid Antibacterial activity |
| Zdroj: | JPC - Journal of Planar Chromatography - Modern TLC. 31:355-359 |
| ISSN: | 1789-0993 0933-4173 |
| Popis: | In this study, thin-layer chromatography—contact bioautography (TLC—CB) was used for the bioassay-guided isolation of antibacterial compounds against Streptococcus mutans from ethanolic extract of Pinus merkusii heartwood. In the TLC—CB technique, clear inhibition zones at two major spots on the TLC plate were observed against S. mutans. The major separated anti-S. mutans compounds were isolated by a preparative TLC plate (silica gel) with the mobile phase composed of n-hexane and ethyl acetate (7:3 v/v). The isolated components were identified as dehydroabietic acid and resin acids. Resin acids were further purified by a pentafluorophenyl (PFP) high-performance liquid chromatography (HPLC) column using water and methanol as eluents. Isopimaric acid and abietic acid were obtained. The antibacterial properties of dehydroabietic acid, isopimaric acid, and abietic acid against four strains of S. mutans were evaluated using broth microdilution method. The results revealed that the isolated substances exhibited antibacterial activity against all bacterial strains. |
| Databáze: | OpenAIRE |
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