Use of a quality-control plasma sample to decrease interassay variation in radioimmunoassays of apolipoprotein A-I

Autor:	Bruce A. Kottke, Yin Ping T. Au, B. J. Hallaway
Rok vydání:	1986
Předmět:	Chromatography Plasma samples Apolipoprotein B biology medicine.diagnostic_test Chemistry Biochemistry (medical) Clinical Biochemistry Radioimmunoassay Apolipoproteins A Antigen Immunoassay Immunology Blood plasma biology.protein medicine lipids (amino acids peptides and proteins) Lipoprotein
Zdroj:	Clinical Chemistry. 32:1394-1397
ISSN:	1530-8561 0009-9147
Popis:	The apolipoprotein A-I (apo A-I) radioimmunoassay established in our laboratory involves use of purified apo A-I as the primary standard for quantifying apo A-I in plasma and a pooled plasma (quality-control sample) as a secondary standard to decrease interassay variation. The measured values for apo A-I increased over time as the labeled antigen degraded. We observed these results in two separate studies: (a) apo A-I in plasma from 16 subjects was measured 12 times during four months, and (b) apo A-I in a single pooled plasma was measured 48 times during 10 months. We show that use of a quality-control plasma sample as a secondary standard decreased interassay variation, which was, in part, ascribable to degradation of the labeled antigen.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fa9fda1642c325ba00c82fc3e325c0e0 https://doi.org/10.1093/clinchem/32.7.1394 Zobrazit plný text záznamu