| Popis: |
Fungal endophytes associated with medicinal plants are of research interests, since they provide a viable alternative source for molecules with medicinal value. In this study, we report for the first time two fungal endophytic isolates, AI.EF 001 and AI.EF 002 belonging to the genus Daldinia from the leaves of medicinal plant, Abutilon indicum linn (AI). Both AI.EF were identified as Daldinia eschscholtzii (DE) species by ITS1-5.8-ITS2 sequence analysis and phylogenetic tree reconstruction by Neighbor-joining method. Crude extracts of DE (EFEA), generated by ethylacetate/water fractionation of the fungal methanol extracts when subjected to column chromatography separation yielded 5 compounds. NMR and other spectral data revealed the compounds to be ɑ-napthoflavone, Syringaldehyde, 3,4,5-trimethoxy benzoic acid, 2-Furoic acid and Gossypetin 3′ O glycoside. All of these compounds are being reported for the first time from DE. The isolated compounds showed promising free radical scavenging activities. The compounds also exhibited anti inflammatory property by down regulating intracellular ROS as well as inhibiting LPS induced lipid peroxidation in AtT 20 mouse pituitary cells. Current finding demonstrates endophyte DE as a new source for the flavanoid, Gossypetin-3′-O-glycoside along with other phytoconstituents with strong antioxidant and anti-inflammatory activity. |
