Spleen colony studies of leukemia L1210
| Autor: | Craig Foley, Isidore Wodinsky, Charles J. Kensler |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Pathology
medicine.medical_specialty education.field_of_study Population Spleen General Medicine Biology Leukemia L1210 medicine.disease General Biochemistry Genetics and Molecular Biology Andrology medicine.anatomical_structure Colony assay medicine Bioassay L1210 cells Neoplasm Cell destruction General Agricultural and Biological Sciences education |
| Zdroj: | Cryobiology. 4:333-336 |
| ISSN: | 0011-2240 |
| DOI: | 10.1016/s0011-2240(68)80132-4 |
| Popis: | Summary The spleen colony assay showed that the percentage of viable L1210 cells frozen to −15°C and thawed was similar to that for nonfrozen leukemic cells as estimated by colonyforming units (CFU). At temperatures between −15 and −60°C, the CFU decreased exponentially, demonstrating cellular injury during the freezing or thawing process. The surviving CFU fraction at −60°C was 10% of the nonfrozen controls, and further reduction of the temperature to 195°C resulted in no further increase of cell destruction. |
| Databáze: | OpenAIRE |
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