Organophosphate hydrolase conjugated UiO-66-NH2 MOF based highly sensitive optical detection of methyl parathion
| Autor: | Akash Deep, Ashok K. Paul, Sarita Dhaka, Siddavattam Dayananda, Jyotsana Mehta |
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| Rok vydání: | 2019 |
| Předmět: |
Detection limit
Chromatography Chemistry Organophosphate 010501 environmental sciences 01 natural sciences Biochemistry 03 medical and health sciences Hydrolysis chemistry.chemical_compound 0302 clinical medicine Parathion Hydrolase Parathion methyl 030212 general & internal medicine Biosensor 0105 earth and related environmental sciences General Environmental Science Conjugate |
| Zdroj: | Environmental Research. 174:46-53 |
| ISSN: | 0013-9351 |
| DOI: | 10.1016/j.envres.2019.04.018 |
| Popis: | The hexahistidine-tagged organophosphorus hydrolase (OPH6His) has been immobilized on a Zr-MOF, namely UiO-66-NH2. The resulting enzyme-MOF composite was used as a carrier to facilitate the hydrolysis of an organophosphate pesticide, i.e., methyl parathion in to p-nitrophenol (PNP). The formation of PNP took place in direct proportion to the added pesticide concentration. Coumarin1 (7-diethylamino-4-methylcoumarin) was then introduced in the reaction mixture as a reporter fluorescent molecule. As PNP acted to quench the fluorescence of coumarin1, it became possible to detect methyl parathion over a wide concentration range of 10–106 ng/mL with an achievable limit of quantification as 10 ng/mL. The immobilization of OPH6His on the surface of UiO-66-NH2 was found to endow an improvement in the enzymatic activity by about 37%. The OPH6His/UiO-66-NH2 conjugate was reusable for at least up to eight times and also found stable toward long-term storage (minimum 60 days). The potential practical utility of the above proposed sensing method has been demonstrated by employing it for an accurate analysis of pesticide-spiked food samples. |
| Databáze: | OpenAIRE |
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