Enhancing forensic efficiency of STR typing using mass spectrometry-based assay

Autor:	Tatyana Timoshenko, Elena Zemskova, Ivanov Pl, Sergey Leonov
Rok vydání:	2013
Předmět:	Genetics STR multiplex system Pcr cloning SNP Locus (genetics) Str typing Typing Biology Allele Mass spectrometry Pathology and Forensic Medicine
Zdroj:	Forensic Science International: Genetics Supplement Series. 4:e280-e281
ISSN:	1875-1768
Popis:	Using the Abbott PLEX-ID PCR/mass spectrometry-based STR assay covering a total of 13 CODIS loci, we typed 310 blood samples from unrelated Russian individuals all had full STR profiles generated with the conventional ABI Identifier Plus/CE system. The MS platform provides for determination of PCR product base compositions thus enabling to determine when an allele has a SNP within the amplified region. Sequence-based polymorphisms relative to the reference alleles were seen in 10 of the 13 loci tested. Up to 15 variant alleles in particular locus were observed. At the 8 loci at least one representative heterozygous individual was found for which the locus is called homozygous by conventional typing. The results demonstrate potential advantages of the sequence-based polymorphisms assay in resolution of samples when limited number of loci can be STR-typed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fa5896b0f7a4054b6269f0dcd9c188d1 https://doi.org/10.1016/j.fsigss.2013.10.143 Zobrazit plný text záznamu Full Text from ScienceDirect