ENZYMATIC PRODUCTION OF FRUCTOOLIGOSACCHARIDES FROM PHYTOENZYMES OF ANANAS COMOSUS WASTE BY USING RESPONSE SURFACE METHODOLOGY: ONE FACTOR

Autor:	Mahrus Ali, Ahmad Noormazlinah, Nor Hanimah Hamidi, M.J. Nur Dini, M.A. Nurul Aini, A. M. Mimi Sakinah
Rok vydání:	2018
Předmět:	chemistry.chemical_classification Design–Expert Sucrose biology Prebiotic medicine.medical_treatment Low calorie biology.organism_classification chemistry.chemical_compound Enzyme chemistry medicine Food science Response surface methodology Ananas
Zdroj:	Journal of Chemical Engineering and Industrial Biotechnology. 3:26-36
ISSN:	0126-8139 2600-7347
DOI:	10.15282/jceib.v3i1.3874
Popis:	Fructooligosaccharides (FOS) are low calorie sweeteners with prebiotic property which is widely used as functional food material.FOS can be produced by using phytoenzymes from Ananas comosus waste. Pineapple (Ananas comosus) fruits are available at most of Asean country. In this work, One Factor at A Time (OFAT) by Response Surface Method (RSM) using Design Expert version 7.0 have been applied to optimize the FOS concentrations by enzymatic reaction of sucrose with phytoenzymes from Ananas comosus waste. The studied variables were: temperature (30-90˚C), pH (3-9), time of reaction (10-120 minutes), sucrose concentrations (20%-80%w/v) and phytoenzymes concentrations (10%-100% w/v) of reaction correspondingly. As a result, an optimum condition FOS production was obtained are, time of reaction (100 minutes), temperature (60˚C), pH (5.5), sucrose concentration (60%w/v), and phytoenzymes concentration (30% w/v), respectively and producing 150.456 (g/ml).This investigation can be further extended to explore the prebiotic potential of synthesized FOS.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fa5220284ec6aca2d57000cc975018f5 https://doi.org/10.15282/jceib.v3i1.3874 Zobrazit plný text záznamu