PCR-based clonality analysis: a reliable method for the diagnosis and follow-up monitoring of conservatively treated gastric B-cell MALT lymphomas?
| Autor: | T Diss, D Delia, Antonella Aiello, Silvana Pilotti, Carlo Tondini, M. Balzarotti, Huaizheng Peng, Roberto Giardini |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Pathology
medicine.medical_specialty Histology MALT lymphoma General Medicine Gene rearrangement Biology medicine.disease Pathology and Forensic Medicine Lymphoma law.invention medicine.anatomical_structure law Monoclonal medicine Immunohistochemistry Immunoglobulin heavy chain Polymerase chain reaction B cell |
| Zdroj: | Histopathology. 34:326-330 |
| ISSN: | 0309-0167 |
| DOI: | 10.1046/j.1365-2559.1999.00628.x |
| Popis: | Aims We evaluated polymerase chain reaction (PCR) amplification of specific immunoglobulin heavy chain (IgH) gene rearrangements as a means of demonstrating monoclonality during follow-up of conservatively treated gastric MALT lymphoma, and compared the reproducibility of PCR on sequential frozen and paraffin-embedded endoscopic biopsies. We established an association between clonality detected by PCR and the histological observations. Methods and results Sixty-nine pairs of sequential frozen and paraffin-embedded endoscopic biopsies from 21 conservatively treated patients were graded according to the Wotherspoon–Isaacson histological scoring system, which provides a measure of diagnostic confidence on a scale 0–5. PCR amplification of the IgH gene was performed using FR3/JH and FR2/JH primers. 68/69 paired samples (98.5%) showed identical mono- or polyclonal PCR amplification patterns. Forty-seven out of 48 pairs of samples sharing similar histological features produced identical amplification patterns in both fresh and paraffin-embedded tissues. In comparison with the histological grading, monoclonality was detected in 64.2% and 41.6% of samples scored 5 and 4, respectively. Conversely, among 64 samples scored 0–3, a monoclonal pattern was observed only in two samples, one of which was from a patient who relapsed 9 months later. Conclusions PCR-based clonality analysis by demonstration of specific IgH gene rearrangement can be easily and reliably performed on both frozen and paraffin-embedded endoscopic biopsies. In conjunction with histological observation, this method can be used as a complementary tool to monitor MALT lymphoma regression during conservative treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|