ACAPELLA-5K, a high-throughput automated genome and chemical analysis system
| Autor: | Mohan S. Saini, Deirdre R. Meldrum, Mark R. Holl, Charles H. Fisher, Stephen E. Moody, M.P. Moore, Peter Wiktor, William H. Pence, David L. Cunningham |
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| Rok vydání: | 2004 |
| Předmět: |
Reproducibility
Computer science business.industry Drug discovery Nanotechnology Pipeline (software) Genome DNA sequencing law.invention chemistry.chemical_compound chemistry law Reagent Instrumentation (computer programming) Process engineering business Throughput (business) DNA Polymerase chain reaction |
| Zdroj: | IROS |
| DOI: | 10.1109/iros.2003.1249217 |
| Popis: | A capillary-based fluid handling system bas been developed to process 5000 samples in 8 hours. The system takes deoxyribonucleic acid (DNA) or other chemical samples and automatically processes them as specified in a user-defined protocol with aspiration, dispensing, mixing, thermal cycling, and imaging steps. A serial pipeline process is used to provide flexibility, reproducibility, and reliability for the samples prepared. This laboratory automation system dispenses 40-100 pL droplet volumes using piezoelectric reagent dispensers and prepares 1 or 2-/spl mu/L final reaction volumes, a 3 to 5-fold reduction in reagent usage over current (2003) state-of-the-art manual and automated instrumentation. Extensive testing of the system has been performed with the University of Washington Genome Center. Applications for ACAPELLA-5K include DNA sequencing, diagnostics, minimal residual disease quantification, drug discovery, environmental testing, forensics, protein crystallography, Polymerase Chain Reaction (PCR) and so on. |
| Databáze: | OpenAIRE |
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