Electrochemical determination of hemoglobin, hematocrit, and hemolysis
| Autor: | D R Peterson, H W Levin, R K Kobos, H Kilkson, J W Dickinson, S D Abbott |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Chromatography
medicine.diagnostic_test Biochemistry (medical) Clinical Biochemistry Analytical chemistry chemistry.chemical_element Hematocrit medicine.disease Oxygen Hemolysis Ion selective electrode law.invention chemistry.chemical_compound chemistry law medicine Hemoglobin Clark electrode Fluoride Whole blood |
| Zdroj: | Clinical Chemistry. 33:153-158 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/33.1.153 |
| Popis: | Novel electrochemical methods have been developed for determination of total hemoglobin, hematocrit, and detection of hemolysis in whole blood. Hemoglobin is measured through its peroxidase activity, a fluoride ion-selective electrode being used to monitor the rate of fluoride ion production from the oxidation of an organofluorine compound. Results agree well with those obtained with the cyanmethemoglobin method (r = 0.970). Hematocrit is determined from the ratio of the sodium ion concentrations measured with an ion-selective electrode before and after lysis of the erythrocytes. Results by this and the microhematocrit method correlated well (r = 0.987). Hemolysis in a whole-blood sample is detected by using an oxygen electrode to measure the oxygen released when hemoglobin in plasma is oxidized. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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