Expression of Protein Elicitor-Encoding Gene pemG1 in Tobacco (Nicotiana tobacum cv. Samsun NN) Plants and Enhancement of Resistance to TMV

Autor:	Xiu-Feng Yang, Zeng Hongmei, Yuan Jingjing, De-Wen Qiu, Jianjun Mao
Rok vydání:	2009
Předmět:	biology Tobacco mosaic virus Plant Science biology.organism_classification Agronomy and Crop Science Gene Virology Molecular biology Biotechnology Nicotiana Elicitor
Zdroj:	ACTA AGRONOMICA SINICA. 34:2070-2076
ISSN:	0496-3490
DOI:	10.3724/sp.j.1006.2008.02070
Popis:	通过根癌农杆菌( Agrobactrium tumefaciens )介导转化法，将含有稻瘟菌蛋白激发子基因 pemG1 的植物表达载体pCAMBIA2300-Ubi- pemG1 -Ocs转化三生烟( Nicotiana tobacum cv. Samsun NN)，获得了转基因植株。用PCR检测抗卡那霉素烟苗确认阳性转化株，用Southern、Northern和Western杂交进一步证实了 pemG1 基因的整合、转录和表达。对T2代转基因阳性株进行烟草花叶病毒(Tobacco Mosaic Virus)接种试验。接种5 d后发现，与非转基因对照相比，表达pemG1的烟草叶片枯斑数量减少，表明稻瘟菌蛋白激发子基因 pemG1 的表达提高了转基因烟草对TMV的抗性。
Databáze:	OpenAIRE
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