Abstract 060: Role of Mir-214 in the Regulation of Perivascular Fibrosis in Angiotensin II Induced Hypertension
| Autor: | Aurelie Nguyen Dinh Cat, Dominik Skiba, Laura Denby, Mateusz Siedlinski, Tomasz J. Guzik, Eilidh McGinnigle, Andy Baker, Ryszard Nosalski |
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| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Hypertension. 70 |
| ISSN: | 1524-4563 0194-911X |
| DOI: | 10.1161/hyp.70.suppl_1.060 |
| Popis: | Objective: Hypertension (HT) is associated with perivascular inflammation and increased vascular fibrosis. MicroRNAs (miR) are a novel gene expression regulation mechanism and play a pivotal role in a range of pathological processes. The role and mechanism of miR214 in vascular fibrosis is unknown. Methods: 3-month-old C57BL/6, miR214KO and wild-type littermates were treated with angiotensin II (AngII, 490ng/kg/min; n=6-10) or control buffer for 14 days. PVATs from C57BL/6 animals were analysed using TaqMan_Rodent_microRNA_Arrays. Histological studies, wire myography, lucigenin-enhanced luminometry and cytometrical analysis was conducted, followed by statistical analysis with ANOVA or t-test. Data are expressed as a mean±SEM. Results: Out of 381 miRs, 16 were significantly overexpressed in C57BL/6 AngII animals, with only miR214 showing 8-fold induction (p Conclusions: MiR-214 plays a major role in modulation of aortic fibrosis, vascular function, oxidative stress and perivascular inflammation. |
| Databáze: | OpenAIRE |
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