Separation of two populations of fish hepatocytes by digitonin infusion: some metabolic patterns and hormonal responsiveness
| Autor: | D. Ricci, Elena Fabbri, Antonio Capuzzo, Erika M. Plisetskaya, Gavioli Me, L. Brighenti, C. Ottolenghi, Puviani Ac |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
biology Histology Metabolism biology.organism_classification chemistry.chemical_compound medicine.anatomical_structure Digitonin Endocrinology chemistry Hepatocyte Internal medicine Ictalurus medicine Fish Animal Science and Zoology Ecology Evolution Behavior and Systematics Catfish Hormone |
| Zdroj: | Canadian Journal of Zoology. 69:427-435 |
| ISSN: | 1480-3283 0008-4301 |
| DOI: | 10.1139/z91-067 |
| Popis: | Two pools of liver cells, enriched either in periportal or in perivenous hepatocytes, were obtained from the catfish Ictalurus melas by the selective destruction of the liver by digitonin infusion. Following digitonin pulses directed either orthograde via the portal vein or retrograde via the hepatic vein, hepatocytes from undamaged perivenous and periportal zones, respectively, were isolated by the conventional collagenase perfusion procedure. Eluates from either periportal or perivenous zones were collected for a total of 9 min before, during, and immediately following the digitonin pulse. The eluates were assayed for protein, glycogen, glucose, and activities of lactate dehydrogenase and alanine aminotransferase, and their yield ("recovery") was estimated as a percentage of the total hepatic content. The aforementioned metabolic compounds and enzyme activities were measured also in periportal and perivenous liver cells. No significant differences were obtained between these cells. Both pools of hepatocytes were more than 90% viable and remained hormone responsive. Epinephrine (3 × 10−8 M) or salmon glucagon (3 × 10−8 M) elevated cAMP content, and stimulated glycogen phosphorylase a activity, glycogen depletion, and glucose release in periportal and perivenous liver cells. No differences were observed between periportal and perivenous cells in their metabolic responsiveness to either hormone. |
| Databáze: | OpenAIRE |
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