Study of 17β-estradiol-3-benzoate, 17α-methyltestosterone and medroxyprogesterone acetate fixation in bovine hair
| Autor: | François Andre, Nora Cesbron, Bruno Le Bizec, Emmanuelle Bichon, Lauriane Rambaud |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Chromatography
integumentary system Trimethylsilyl Anabolism Silylation medicine.medical_treatment Extraction (chemistry) Mass spectrometry Biochemistry Dithiothreitol Analytical Chemistry Steroid chemistry.chemical_compound chemistry medicine Environmental Chemistry Methyltestosterone Spectroscopy medicine.drug |
| Zdroj: | Analytica Chimica Acta. 532:165-176 |
| ISSN: | 0003-2670 |
| Popis: | The detection of steroid residues in hair is a powerful strategy to demonstrate long-term administration of these growth promoters in meat production animals. A fast and reliable method was developed for monitoring anabolic steroids and their esters in hair. A 100 mg hair sample was converted into powder and extracted at 50 °C with methanol (sebum fraction). The remaining hair was digested with 1 M NaOH for further extraction of bound steroids. The two fractions were separately purified onto an aminopropyle solid-phase extraction column and onto a silica SPE cartridge. Steroids were detected either by gas chromatography–tandem mass spectrometry after silylation using N-methyl-N-(trimethylsilyl)-trifluoroacetamide/trimethyliodosilane/dithiothreitol or liquid chromatography–tandem mass spectrometry. This method was applied to hair samples collected over a three months period after treatment of three cows respectively with 17α-methyltestosterone, medroxyprogesterone acetate and 17β-estradiol-3-benzoate. The fixation kinetic into hair of the three steroids have been deeply examined and discussed; relation in-between concentration and distance from the injection site, influence of hair colour and sample treatment consequences have been discussed. |
| Databáze: | OpenAIRE |
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