Determination of Sulfonamides in Triplesulfa Cream by Hplc Method
| Autor: | Aqeel A. Fatmi, S. Z. Masih, Denise Taylor |
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| Rok vydání: | 1984 |
| Předmět: | |
| Zdroj: | Drug Development and Industrial Pharmacy. 10:31-43 |
| ISSN: | 1520-5762 0363-9045 |
| DOI: | 10.3109/03639048409038290 |
| Popis: | A fast, accurate, economical, and reproducible HPLC procedure has been developed to quantitate sulfacetamide (I), sulfabenzamide (II), and sulfathiazole (III) in vaginal creams, using sulfapyridine (IV) as internal standard. The method employed u Bondapak phenyl column, Waters Liquid Chromatograph, Hewlett-Packard 3390A reporting integrator and UV detector set at 280 nm. The mobile phase consisted of 7:3 mixture of 0.01 M ammonium hydrogen phosphate and methanol (pH 7.2). The method met USP requirements for system suitability and linearity (R=0.998). The USP method consistently gave low and widely varying results ranging from 75 to 102% for sulfacetamide. The results for sulfabenzamide and sulfathiazole were comparable by both methods. The relative retention time for I, II, III, IV were 0.4, 0.5, 0.9, and 1.0 respectively for the proposed method and 0.8, 2.5, 1.8, and 1.0 for the USP method. The USP method was tedious and unreliable for assay of (I). Considering the cost of the column and mobile p... |
| Databáze: | OpenAIRE |
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