Alterations in glucose metabolism in chick embryo cells transformed by Rous sarcoma virus
| Autor: | Malathy Singh, B.L. Horecker, J.T. August, Vishwa Nath Singh |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
chemistry.chemical_classification
Rous sarcoma virus Hexokinase animal structures Biophysics Dehydrogenase Biology biology.organism_classification Biochemistry Molecular biology chemistry.chemical_compound Enzyme chemistry Lactate dehydrogenase embryonic structures Glycolysis Molecular Biology Pyruvate kinase Phosphofructokinase |
| Zdroj: | Archives of Biochemistry and Biophysics. 165:240-246 |
| ISSN: | 0003-9861 |
| Popis: | Effects of transformation by Rous sarcoma virus of Schmidt-Ruppin strain on the activities of key enzymes of the glycolytic and the hexose monophosphate shunt pathways in chick-embryo cells were investigated. Activities of hexokinase, phosphofructokinase, pyruvate kinase, lactate dehydrogenase, and glucose-6- P dehydrogenase were increased about twofold in the transformed cells, but that of 6- P -gluconate dehydrogenase remained unaltered. The transformation-mediated increase in the activity of hexokinase was confined entirely to the bound form of the enzyme. Cells infected with a temperature-sensitive mutant (Ts-68) of Schmidt-Ruppin strain of Rous sarcoma virus showed the typical increase in the rate of 2-deoxyglucose uptake and the activities of hexokinase, phosphofructokinase, pyruvate kinase, and glucose-6- P dehydrogenase at the permissive temperature (37 °C), but when the infected cells were grown at the nonpermissive temperature (41 °C), the increases in the sugar uptake and activities of these enzymes were abolished. Unlike the regulatory enzymes, lactate dehydrogenase activity was increased at both the permissive and the nonpermissive temperatures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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