COMPARATIVE EVALUATION OF ANTIBACTERIAL EFFICACY OF CHLOROQUICKONE STEP IRRIGATING SOLUTION AND CHLORHEXIDINE GLUCONATE SOLUTION ON E. FAECALIS IN ROOT CANAL SYSTEM OF PRIMARY MOLARS: A RANDOMISED EX VIVO STUDY
| Autor: | Dave Bhavna H, Shah Paridhi S |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Molar
Chemistry business.industry Root canal 0206 medical engineering Dentistry 030206 dentistry 02 engineering and technology Antibacterial efficacy 020601 biomedical engineering Comparative evaluation 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure medicine Chlorhexidine Gluconate Solution business Ex vivo |
| Zdroj: | INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH. :1-3 |
| DOI: | 10.36106/ijsr/0615389 |
| Popis: | Background: Microorganisms and their by-products are considered to be primary etiologic agents in endodontic diseases. Mechanical preparation is the principal mode of reducing the bacterial population in the root canal system, smear layer is still left on the root canal wall. Hence, irrigation of root canal allows for cleaning beyond what might be achieved by root canal instrumentation allows. Aim: To evaluate and compare antibacterial efficacy of Chloroquick- one step irrigating solution and 2% Chlorhexidine Gluconate on E. faecalis in primary molars by Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) study. Methodology: 40 children between age 4-7 years were included in this ex- vivo study based on selection criteria. In Group I (n=20) irrigation was done with chloroquick and normal saline and in Group II (n=20) irrigation was done with 2% chlorhexidine gluconate and normal saline. Pre- and post-irrigation samples were collected using sterile paper points and transferred into transporting medium in Eppendorf tube and sent for qRT-PCR analysis. Results: On intra-group comparison, in Group I a statistically significant reduction in E. Faecalis (colony forming units/ml) count post-irrigation was found (p=0.0130). While for Group II, there was no statistically significant reduction in E. Faecalis (colony forming units/ml) count post-irrigation (p=0.8180). On inter-group comparison between, Group I showed statistically significant reduction in E. Faecalis (colony forming units/ml) count as compared to Group II (p=0.0110) Conclusion: In the present study, continuous soft chelating irrigation protocol has shown promising results for reduction of microbial load from the root canal system of primary teeth |
| Databáze: | OpenAIRE |
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