Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer
| Autor: | Yasin Ina-Salwany, Aslah Mohamad, Nurhidayu Al-saari, Yong Kit Chin, Mohd Zamri-Saad, Annas Salleh, Zarirah Zulperi, Santha Silvaraj, JingYie Lee, Aslizah Mohd-Aris |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Gill
0303 health sciences Kidney Veterinary medicine business.industry Vibrio harveyi Spleen 04 agricultural and veterinary sciences Aquatic Science Biology biology.organism_classification Mucus Lates Vaccination 03 medical and health sciences medicine.anatomical_structure Aquaculture 040102 fisheries medicine 0401 agriculture forestry and fisheries business 030304 developmental biology |
| Zdroj: | Aquaculture Research. 51:389-399 |
| ISSN: | 1365-2109 1355-557X |
| DOI: | 10.1111/are.14386 |
| Popis: | Vibrio harveyi causes vibriosis in various marine aquaculture fish species, especially when they are young. The infection subsequently leads to significant economic losses for aquaculture farms. Vaccination is recommended to control this disease. This study describes the efficacy of a live attenuated V. harveyi strain MVh_vhs (LAVh) as a vaccine candidate in controlling infection by wild‐type V. harveyi (WTVh) in Lates calcarifer. A total of 240 fingerlings were divided into four groups. Group 1 was not vaccinated and was not challenged, Group 2 was vaccinated with a formalin‐killed V. harveyi (FKVh), Group 3 was vaccinated with the LAVh before challenge and Group 4 was not vaccinated and was challenged. Bath vaccination was employed for one hour before the LAVh distribution was determined in the fish mucus, gill, liver, gut, kidney and spleen. The gills, livers, kidneys and skins were also sampled for gene expression analysis. To challenge the fish, skin abrasion was conducted before the fish were challenged by immersion with WTVh. The results revealed an extensive distribution of the LAVh in the liver and kidneys of the fish in Group 3 for the first 12 hr, resulting in mild lesions compared with Group 1. Similarly, there were significantly (p |
| Databáze: | OpenAIRE |
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