One-step sandwich enzyme immunoassay for serum myoglobin with three monoclonal antibodies to different epitopes
| Autor: | M. D. Smirnova, Yu. S. Massino, G. I. Kolyaskina, Kizim Ea, Dmitriev Ad, Segal Ol, Avilov Vv, Saprygin Db, Smotrov Sp, Nikulina Va |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
inorganic chemicals
biology medicine.drug_class Serum myoglobin One-Step General Medicine Monoclonal antibody Molecular biology Horseradish peroxidase General Biochemistry Genetics and Molecular Biology Epitope chemistry.chemical_compound Myoglobin chemistry biological sciences biology.protein medicine Sandwich enzyme immunoassay Antibody |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 127:544-546 |
| ISSN: | 1573-8221 0007-4888 |
| Popis: | Enzyme-linked sandwich immunosorbent assay for serum myoglobin with 3 monoclonal antibodies to different epitopes is described. Antibodies 13G6 and 18H2 were adsorbed on a carrier, and 14D6 antibodies were labeled with horseradish peroxidase. One-step incubation with standard myoglobin solution yielded double sandwich: adsorbed antibodies—myoglobin—labeled antibodies; the intensity of peroxidase reaction was directly proportional to myoglobin concentration. The sensitivity of the proposed method for serum myoglobin was below 5 ng/ml. The sensitivity of the method increases due to myoglobin binding with two antibodies adsorbed on the carrier. Fluctuations within and between series did not exceed 10%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink