Anabolic Steroids do not Change mRNA Levels and Protein Secretion of Apolipoprotein A-I and B-100 in HepG2 Cells
| Autor: | W. März, H. Sartor, G. Wolter, W. Gross, K. J. Lackner |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Apolipoprotein B biology Anabolism Chemistry Cholesterol medicine.medical_treatment chemistry.chemical_compound Endocrinology High-density lipoprotein Internal medicine Gene expression medicine biology.protein lipids (amino acids peptides and proteins) Secretion Anabolic steroid Stanozolol medicine.drug |
| Zdroj: | Recent Developments in Lipid and Lipoprotein Research ISBN: 9783540559955 |
| Popis: | The effects of two anabolic steroids, stanozolol and methenolone, on gene expression and protein secretion of apolipoprotein A-I and apolipoprotein B-100 were analyzed in the human hepatoma cell line HepG2. Concentrations of mRNA for apolipoproteins A-I and B-100 were determined by quantitative slot blot hybridization. The amount of apolipoproteins secreted into the media were measured by zone immuno electrophoresis or enzyme linked immunosorbent assay. At concentrations of 10–9 M to 10–7 M no effect on mRNA concentration or apolipoprotein secretion could be detected. At higher concentrations of the anabolic steroids non-specific toxic effects on the HepG2 cells were observed. It is concluded that the known effects of anabolic steroids on lipoprotein metabolism in vivo, i.e. lowering of high density lipoprotein cholesterol and apolipoprotein A-I and increase of low density lipoprotein cholesterol and apolipoprotein B-100, is not initiated by significant changes in synthesis and secretion of the respective apolipoproteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|