Visualization of allergic immunity reveals non-coordinate expression of IL-4 and IL-13 in the innate immune system (HYP6P.260)
| Autor: | Thomas O'Brien, Katherine Bao, Wei Xin Gladys Ang, Soman Abraham, Richard Reinhardt |
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| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 192:118.5-118.5 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.192.supp.118.5 |
| Popis: | Allergic disease affects more than 3 billion people globally. In developed countries, allergic disease manifests itself as allergy and asthma, while in developing countries it takes the form of parasitic infections. The pathogenesis that develops as a consequence of allergic inflammation is mediated by the cytokines interleukin-4 (IL-4) and IL-13. Re-stimulation studies have demonstrated that a variety of cells in the lung are competent to produce IL-4 and IL-13 in vitro. At the present time, however, we have no clear understanding of which cells are responsible for producing these cytokines in vivo. In order to examine the cellular sources of IL-4 and IL-13 during allergic inflammation, we used IL-4 and IL-13 cytokine reporter mice to directly visualize cytokine production in vivo. These studies revealed that CD4+ T cells were the primary source of both IL-4 and IL-13 in allergic inflammation. Additionally, these studies revealed that CD4+ T cells produce IL-4 and IL-13 equivalently on a per cell basis. This is in stark contrast to innate cells, which preferentially produce either IL-4 or IL-13. NKT cells, eosinophils, and basophils produce IL-4, while ILC2 cells produce IL-13. Interestingly, IL-13 production was restricted to cells expressing high GATA-3. No such relationship was observed in IL-4 producing innate cells. In summary, these findings challenge the current dogma of coordinate regulation of IL-4 and IL-13 during allergic inflammation. |
| Databáze: | OpenAIRE |
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