Type 1 interferon-dependent repression of NLRC4 and iPLA2 licenses down-regulation of Salmonella flagellin inside macrophages
| Autor: | Bhavana S. Lakshmi, Naeha Subramanian, Ajay Suresh Akhade, Neha Dikshit, Ayub Qadri, Kelly T. Hughes, Shaikh M. Atif |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Salmonella Multidisciplinary biology Pyroptosis Inflammasome medicine.disease_cause Microbiology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine NLRC4 medicine biology.protein Macrophage Pathogen Intracellular Flagellin 030215 immunology medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 117:29811-29822 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Inflammasomes have been implicated in the detection and clearance of a variety of bacterial pathogens, but little is known about whether this innate sensing mechanism has any regulatory effect on the expression of stimulatory ligands by the pathogen. During infection with Salmonella and many other pathogens, flagellin is a major activator of NLRC4 inflammasome-mediated macrophage pyroptosis and pathogen eradication. Salmonella switches to a flagellin-low phenotype as infection progresses to avoid this mechanism of clearance by the host. However, the host cues that Salmonella perceives to undergo this switch remain unclear. Here, we report an unexpected role of the NLRC4 inflammasome in promoting expression of its microbial ligand, flagellin, and identify a role for type 1 IFN signaling in switching of Salmonella to a flagellin-low phenotype. Early in infection, activation of NLRC4 by flagellin initiates pyroptosis and concomitant release of lysophospholipids which in turn enhance expression of flagellin by Salmonella thereby amplifying its ability to elicit cell death. TRIF-dependent production of type 1 IFN, however, later represses NLRC4 and the lysophospholipid biosynthetic enzyme iPLA2, causing a decline in intracellular lysophospholipids that results in down-regulation of flagellin expression by Salmonella These findings reveal a previously unrecognized immune-modulating regulatory cross-talk between endosomal TLR signaling and cytosolic NLR activation with significant implications for the establishment of infection with Salmonella. |
| Databáze: | OpenAIRE |
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