Noninvasive pH monitoring for bacterial detection in platelet concentrates
| Autor: | Ramie Qaisar, Yuntong Kou, Maria Loza-Correa, Heather Perkins, Steven J. Geelhood, Dilini Kumaran, Sandra Ramirez-Arcos |
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| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
biology Chemistry Immunology Bacillus cereus Hematology Buffy coat 030204 cardiovascular system & hematology Contamination biology.organism_classification Ph monitoring Surgery 03 medical and health sciences 0302 clinical medicine Apheresis Staphylococcus epidermidis medicine Immunology and Allergy Platelet Food science Bacteria 030215 immunology |
| Zdroj: | Transfusion. 56:1348-1355 |
| ISSN: | 0041-1132 |
| DOI: | 10.1111/trf.13557 |
| Popis: | BACKGROUND Bacterial contamination of platelet concentrates (PCs) remains the prevalent posttransfusion infectious risk. The pH SAFE system, a noninvasive method used to measure pH of PC for quality control, was evaluated herein as a rapid method to detect bacterial contamination in PCs. STUDY DESIGN AND METHODS Pairs of ABO-D–matched apheresis and buffy coat PCs were pooled and split into two pH SAFE platelet bags. One of the bags served as the control unit, while the other was inoculated with one of nine clinically relevant bacteria (target concentration approx. 1 colony-forming units [CFUs]/mL). The pH of both PCs was measured over 7 days of storage at approximately 4-hour intervals during daytime. One-milliliter samples were taken at the testing points to determine bacterial concentration. RESULTS PCs with pH values of less than 6.6 or with a pH change over time (ΔpH/Δtime) greater or equal than 0.046 pH units/hr are suspected of being contaminated. pH decreased significantly during storage in all bacterially inoculated PC at concentrations of more than 107 CFUs/mL (p |
| Databáze: | OpenAIRE |
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