Application of Non-Fluorescent Dyes to Assess the Antischistosomal Effect of Antimalarial Drugs on Schistosoma mansoni Adult Worms
| Autor: | Kentaro Kato, Yoshinori Mitsui |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Neutral red Primaquine biology 030231 tropical medicine General Medicine Amodiaquine 030108 mycology & parasitology Pharmacology biology.organism_classification Staining Praziquantel 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Infectious Diseases chemistry Artesunate parasitic diseases medicine Trypan blue Schistosoma mansoni medicine.drug |
| Zdroj: | Japanese Journal of Infectious Diseases. 71:382-387 |
| ISSN: | 1884-2836 1344-6304 |
| Popis: | The possible emergence of praziquantel (PZQ)-tolerant and/or -resistant schistosomes requires the study and development of new antischistosomal drugs as alternatives to PZQ. The present study investigates the capability of 3 dyes-methylene blue (MB), neutral red (NR), and trypan blue (TB)-to assess the in vitro antischistosomal effect of antimalarial drugs on Schistosoma mansoni adult worms. S. mansoni adult worms were incubated in the medium alone as the control or in the medium supplemented with 10 μg/ml primaquine (PQ), artesunate (AR), or amodiaquine (AQ) for 5 days. Viabilities of the worms were observed following staining with MB, NR, or TB. The disparity of MB and NR staining among male and female adult worms treated with PQ, AR, and AQ correlated with the various levels of damage to the male and female worms. Furthermore, the severity of the damage to the adult worms treated with the 3 drugs appeared to be reflected in the TB staining status. The results indicate that the 3 non-fluorescent dyes can serve as useful complementary tools to assess the antischistosomal effect of antimalarial drugs. |
| Databáze: | OpenAIRE |
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