Enhanced Loading and Stability of Urease Covalently Attached to Polypyrrole Microspheres for Application to Urea Biosensor

Autor: Anamika Gambhir, B. Miksa, Arun Kumar, Bansi D. Malhotra, S. Slomkowski
Rok vydání: 2003
Předmět:
Zdroj: New Horizons in Biotechnology ISBN: 9789048164684
DOI: 10.1007/978-94-017-0203-4_14
Popis: An electrode for measurement of urea, based on urease covalently attached to polypyrrole (PPY) microspheres immobilized on conducting polypyrrole polyvinyl sulphonate (PPY-PVS) films has been described. Conducting polypyrrole polyvinyl sulphonate films were prepared using indium-tin-oxide (ITO) glass plates as working electrode and standard calomel electrode as the reference electrode. The films were deposited on ITO glass plates for 5–7 minutes at a constant current of 2mA (surface area 1x1cm2). Urease immobilized onto polypyrrole microspheres (by reaction of protein amino groups with microsphere surface aldehyde groups of the surface of microspheres) was entrapped/adsorbed on electrochemically prepared conducting polypyrrole polyvinyl sulphonate films deposited on ITO. Potentiometric measurements were taken in different concentrations of urea using an ammonium ion analyser.
Databáze: OpenAIRE