RNAseq analysis reveals the role of secondary metabolism in the response of URS 21, a race-nonspecific resistant cultivar, to crown rust
| Autor: | J. L. Gonzalez-Hernandez, Marcelo Teixeira Pacheco, Carla Andréa Delatorre, P. Swaminathan, G. L. de Morais, R. N. Reese, Frank Guzman, A. de A. Figueiró |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics food.ingredient biology food and beverages Plant Science Horticulture biology.organism_classification 01 natural sciences Deep sequencing 03 medical and health sciences 030104 developmental biology Puccinia coronata Avena food Seedling Botany Cultivar Secondary metabolism Agronomy and Crop Science Pathogen Gene 010606 plant biology & botany |
| Zdroj: | Plant Pathology. 66:702-712 |
| ISSN: | 0032-0862 |
| DOI: | 10.1111/ppa.12615 |
| Popis: | Crown rust (Puccinia coronata Corda f. sp. avenae) can devastate oats (Avena sativa). Plants possessing durable resistance are desirable and they may possess diverse mechanisms from those underlying genes with specific effects. Gathering information about the molecular mechanisms involved, especially those not associated to hypersensitive reaction, and combining those acting at different infection stages may increase the ability to detain the pathogen. The cultivar URS 21 has strongly reduced the intensity and speed of the disease for the last 14 years, demonstrating durable race nonspecific resistance expressed at adult and seedling stages. The main objective of this study was to identify by deep sequencing oat transcripts induced by P. coronata inoculation in the URS 21 cultivar at seedling stage. Two main responses to inoculation were further studied, secondary metabolism and oxidative response. The expression of specific genes associated to biosynthesis of secondary metabolites was also evaluated in adult plants by RT-PCR, as well as the accumulation of saponins and avenanthramides due to their recognized importance in other pathosystems comparison being made with reference to the susceptible cultivar URS 22. The URS 21 resistance mechanism probably involves several components, among them, the biosynthesis of avenanthramides and steroid saponins. Several genes related to antioxidative stress were down regulated. Moreover, a large number of oat sequences were generated by RNA-seq analysis. These will help in the search for genes involved in the race nonspecific resistance, and can be useful in the development of markers to search for QTLs associated to race nonspecific resistance. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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