Relationship between expression of Bcl-2 and p53 protein and CagA+ Helicobacter pylori in gastric cancer
| Autor: | Bao-Jie Li, Rui-Bo Zhao, Ya-Ju Du, Jing Zhao |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
biology Atrophic gastritis business.industry Cancer Rapid urease test Disease Helicobacter pylori bacterial infections and mycoses medicine.disease biology.organism_classification Gastroenterology digestive system diseases Internal medicine medicine biology.protein CagA Immunohistochemistry Antibody business |
| Zdroj: | World Chinese Journal of Digestology. 11:554-557 |
| ISSN: | 2219-2859 |
| DOI: | 10.11569/wcjd.v11.i5.554 |
| Popis: | AIM:The relationship between cytotoxin-associated protein (CagA)+Helicobacter pylori (H.pylori) infection and gastric cancer in China was reported inconsistently. Using enzyme-linked immunosorbent assay (ELISA) to detect serum anti-CagA antibody and ABC immunohistochemical staining to detect the expression of Bcl-2 and p53 proteins, this study is to explore the relationship between CagA H.pylori infection and expressions of p53 and Bcl-2 proteins in gastric cancer and chronic gastric diseases. METHODS:Seventy-nine patients (50 with gastric cancer, 17 with chronic atrophic gastritis, 5 with gastric ulcer and 7 with chronic superficial gastritis) were diagnosed by endoscopy and biopsied under endoscopy for detection of H.pylori infection, and confirmed by pathological examination. Expression of Bcl-2 and p53 proteins was detected by immunohistochemistry. Rapid urease test and serum H.pylori antibodies with ELISA or Warthin-Starry silver stains were used for H.pylori diagnosis. H.pylori was defined as positive when 2 or 3 of these tests were positive. ELISA was used for the detection of serum anti-CagA antibody. RESULTS:Anti-CagA antibody was present in 43/50 (86 %) gastric cancer and in 13/29 (41 %) chronic gastric disease, CagA+ H.pylori rate in gastric cancer was higher than those in chronic gastric diseases (P 0.05). In CagA+ gastric cancer it was higher than that in CagAone (65 % vs 43 %, P >0.05), and in CagA+ chronic gastric diseases it was higher than that in CagAone (77 % vs 12 %, P |
| Databáze: | OpenAIRE |
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