Protein adsorption at liquid/liquid interface with low interfacial tension
| Autor: | R. Borbás, Éva Kiss |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Ammonium sulfate
Aqueous solution Chromatography biology Chemistry Surfaces and Interfaces General Medicine Surface tension chemistry.chemical_compound Colloid and Surface Chemistry Adsorption Chemical engineering Emulsion Protein purification biology.protein Physical and Theoretical Chemistry Bovine serum albumin Biotechnology Protein adsorption |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 31:169-176 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/s0927-7765(03)00136-x |
| Popis: | Adsorption of proteins at the interface of two-liquid systems composed of aqueous ammonium sulfate solution and tert-butanol by phase separation was investigated by drop shape tensiometry. The change of interfacial tension with time and protein concentration as well as upon compression of the adsorbed layer were compared for bovine serum albumin, ovalbumin, β-lactoglobulin, lysozyme, trypsin and horse radish peroxidase. A correlation between the dilatational moduli of various protein films and the partitioning of proteins in the two-liquid system was found, which provides evidence to the role of emulsion stability in protein separation by three-phase partitioning. |
| Databáze: | OpenAIRE |
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