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Two new ursane-type triterpene saponins, 3- O -β- d -glucopyranosyl(1 → 3)-[α- l -rhamnopyranosyl(1 → 2)]-α- l -arabinopyranosylurs-12,19(29)-dien-28-oic acid 28- O -α- l -rhamnopyranosyl(1 → 2)-β- d -glucopyranosyl ester ( 1 ) and 3- O -β- d -glucopyranosyl(1 → 3)-[α- l -rhamnopyranosyl(1 → 2)]-α- l -arabinopyranosyl-19α,20α-dihydroxyurs-12-en-28-oic acid 28- O -α- l -rhamnopyranosyl(1 → 2)-β- d -glucopyranosyl ester ( 2 ), along with thirteen known triterpene saponins were isolated from the n -BuOH part of the MeOH extraction of the leaves of Ilex kudingcha C.J. Tseng (also called “Ku-Ding-Cha”). The structures of new compounds were elucidated on the basis of detailed spectroscopic analysis, including HR-ESI-TOF-MS, 1D and 2D-NMR experiments, and by acid hydrolysis. All the compounds were screened for antiplatelet aggregation activity in vitro , and compounds 1 , 2 , 3 , 7 , 12 and 15 showed significant inhibition of platelet aggregation induced by ADP (5 μM) with IC 50 values of 14.7 ± 3.7, 11.3 ± 2.5, 17.4 ± 4.6, 20.5 ± 3.1, 8.1 ± 1.5 and 18.9 ± 4.2 μM, respectively. |
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