Evaluation of antitularaemia immunity via whole human blood leukocyte cytofluorometric analysis

Autor:	Victoria V. Firstova, Tatyana P. Schmelkova, Alexander L. Kravtsov, Tatyana N. Shchukovskaya
Rok vydání:	2005
Předmět:	medicine.diagnostic_test Acridine orange Biology biology.organism_classification Molecular biology In vitro Chromatin Flow cytometry chemistry.chemical_compound chemistry Immunity Leukocyte degranulation medicine Francisella tularensis Whole blood
Zdroj:	SPIE Proceedings.
ISSN:	0277-786X
DOI:	10.1117/12.634771
Popis:	Tularaemia is followed by development of a long-lasting protective immunity. Peripheral blood leukocytes of vaccinated individuals partially lysed in vitro after exposure to killed Francisella tularensis cells and this leukocytolis reaction is used for evaluation of antitularaemia immunity. Here, results from cytofluorometric characterization of human whole blood leukocyte response following exposure to F. tularensis are reported. Leukocytes were stained in whole blood by acridine orange. The green (nuclear chromatin) and red (lysosomal granules) fluorescent signals from individual cells were measured by flow cytometry to detect the decrease of leukocyte concentration in whole human blood (the leukocytolis intensity) and to calculate the damaged leukocytes with changed chromatin structure and lysosomal granules per cell content. Our data indicate that flow cytometry offers a rapid and more informative technique for evaluation of human antitularaemia immunity in vitro.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f97c0806330259f542bc4db9a8fcf46b https://doi.org/10.1117/12.634771 Zobrazit plný text záznamu