Effect of the addition of CMC on the aggregation behaviour of proteins

Autor: H. L. Yu, S.F. Sabato, G D’Aprano, Monique Lacroix
Rok vydání: 2004
Předmět:
Zdroj: Radiation Physics and Chemistry. 71:131-135
ISSN: 0969-806X
DOI: 10.1016/j.radphyschem.2004.04.003
Popis: The effect of carboxymethylcellulose (CMC) on the aggregation of formulation based on calcium caseinate, commercial whey protein (WPC), and a 1:1 mixture of soy protein isolate (SPI) and whey protein isolate (WPI) was investigated. Protein aggregation could be observed upon addition of CMC, as demonstrated by size-exclusion chromatography. This aggregation behaviour was enhanced by means of physical treatments, such as heating at 90°C for 30 min or gamma-irradiation at 32 kGy. A synergy resulted from the combination of CMC to gamma-irradiation in Caseinate/CMC and SPI/WPI/CMC formulations. Furthermore, CMC prevented precipitation in irradiated protein solutions for a period of more than 3 months at 4°C.
Databáze: OpenAIRE