SENSITIVE ANALYSIS OF TETRODOTOXIN IN HUMAN PLASMA BY SOLID-PHASE EXTRACTIONS AND GAS CHROMATOGRAPHY/MASS SPECTROMETRY

Autor:	Hideki Hattori, Osamu Suzuki, Hiroshi Seno, Shunsuke Kurono, T. Yamada
Rok vydání:	2001
Předmět:	Detection limit Chromatography Chemistry Biochemistry (medical) Clinical Biochemistry Extraction (chemistry) Mass spectrometry Biochemistry Analytical Chemistry chemistry.chemical_compound Electrochemistry Gas chromatography Solid phase extraction Gas chromatography–mass spectrometry Derivatization Quantitative analysis (chemistry) Spectroscopy
Zdroj:	Analytical Letters. 34:2439-2446
ISSN:	1532-236X 0003-2719
DOI:	10.1081/al-100107525
Popis:	A method to determine tetrodotoxin (TTX) in human plasma by solid-phase extractions and gas chromatography/mass spectrometry (GC/MS) is described. TTX in plasma samples was passed through Sep-Pak PS-2 extraction cartridges. Then the compound was converted to 2-amino-6-hydroxymethyl-8-hydroxyquinazoline (C9-base) under alkaline conditions and extracted with use of an OASIS HLB extraction cartridge. The C9-base was analyzed after trimethylsilyl derivatization by GC/MS-selected ion monitoring. Recovery rates were 35.3–50.8% and their coefficients of variation were not greater than 16.6%. The calibration curve was linear in the range of 0.5–10.0 ng ml−1 and the detection limit was 0.1 ng ml−1. The coefficients of within-day and day-to-day variations were not greater than 11.8%. Actual determination of TTX in rat plasma after intraperitoneal administration was also presented.
Databáze:	OpenAIRE
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