Direct determination of cyanides by potentiometric biosensors
| Autor: | Michael Keusgen, Joachim P. Kloock, Ingo Krest, Wolfgang Klein, M. Goldbach, Martina Jünger, D.-T. Knobbe, Michael J. Schöning |
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| Rok vydání: | 2004 |
| Předmět: |
Detection limit
Chemistry Cyanide Potentiometric titration Inorganic chemistry Metals and Alloys Electrolyte Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials Metal chemistry.chemical_compound Plating visual_art Materials Chemistry visual_art.visual_art_medium Potentiometric sensor Electrical and Electronic Engineering Instrumentation Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 103:380-385 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2004.04.067 |
| Popis: | Cyanide (CN−) and its derivatives are widely used in the metal mining and metal plating industry. But also many medicinal and food plants are toxic for man because of their content of cyanogenic glycosides. In the present investigation, a cyanide-specific biosensor exploiting immobilized cyanidase (EC 3.5.5.1) has been developed. Enzymatically formed ammonia was either detected by a potentiometric sensor based on an ammonia electrode or by a pH-sensitive electrolyte/insulator/semiconductor (EIS) layer structure made of Al/p-Si/SiO2/Si3N4. It could be demonstrated with both methods that sensitivity of these biosensors is sufficient to detect cyanide in sub-toxic concentrations. The detection limit was determined to be in the micromolar concentration range. |
| Databáze: | OpenAIRE |
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