Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface
| Autor: | Barbara Zavan, Susi Zara, Milena Radunovic, Viviana di Giacomo, Chiara Di Nisio, Vincenzo Luca Zizzari, Adriano Piattelli, José Luis Calvo Guirado, Marianna De Colli, Amelia Cataldi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science medicine.medical_treatment 030206 dentistry Matrix (biology) Bone morphogenetic protein 2 RUNX2 03 medical and health sciences 030104 developmental biology 0302 clinical medicine stomatognathic system In vivo Dental pulp stem cells Ceramics and Composites medicine Implant Dental implant General Dentistry Magnesium ion Biomedical engineering |
| Zdroj: | Dental Materials Journal. 37:197-205 |
| ISSN: | 1881-1361 0287-4547 |
| DOI: | 10.4012/dmj.2016-418 |
| Popis: | Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced. |
| Databáze: | OpenAIRE |
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