Enantioselective esterification reaction using immobilizedCandida rugosa lipase on poly(N-vinyl-2-pyrrolidone-co-styrene) hydrogel
| Autor: | Aiza Harun, Mansor Bin Ahmad, Mahiran Basri, Abu Bakar Salleh |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Chloroform
Materials science Polymers and Plastics biology Triacylglycerol lipase General Chemistry Surfaces Coatings and Films Candida rugosa Styrene chemistry.chemical_compound chemistry Self-healing hydrogels Materials Chemistry biology.protein Organic chemistry 2-Pyrrolidone Lipase Enantiomeric excess |
| Zdroj: | Journal of Applied Polymer Science. 92:3381-3386 |
| ISSN: | 1097-4628 0021-8995 |
| DOI: | 10.1002/app.20342 |
| Popis: | Lipase from Candida rugosa was immobilized on poly(N-vinyl-2-pyrrolidone-co-styrene) hydrogel (poly-(VP-co-ST)) with ethylene dimethacrylate and α,α'-azoisobutyronitrile, which act as crosslinker and initiator, respectively. Three different compositions of monomers were used, namely VP(%):ST(%), 10:90, 50:50, and 70:30 (wt(%)/wt(%)). The immobilized lipases were used in the enantioselective esterification of (R,S)-2-(4-chlorophenoxy)-propanoic acid with n-tetradecanol. The optimum reaction condition of the enantioselective esterification for the native lipase and the poly(VP-co-ST) hydrogel immobilized lipases was determined with respect to temperature, solvents, and initial water activity (aw). The optimum temperature obtained was 40°C, with the poly(VP-co-ST) hydrogel immobilized lipase VP(%)/ST(%):10:90 showing the highest enantiomeric excess. In the solvent effect studies, the best solvents for high enantioselectivity were chloroform and carbon tetrachloride. In the aw studies, optimum αw for NL, VP(%):ST(%), 10:90, and 50:50 was 0.328, while for VP(%):ST(%), 70:30, it was 0.55. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 92: 3381–3386, 2004 |
| Databáze: | OpenAIRE |
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