DNA certification of varieties and hybrids of perennial grass crops

Autor:	I.P. Kondratskaya, V.N. Reshetnikov, V. A. Stolepchenko, P. P. Vasko, O.V. Chizhik, A. N. Yukhimuk
Rok vydání:	2018
Předmět:	Perennial plant Agronomy otorhinolaryngologic diseases food and beverages Certification Biology Hybrid
Zdroj:	Faktori eksperimental'noi evolucii organizmiv. 22:267-273
ISSN:	2415-3826 2219-3782
DOI:	10.7124/feeo.v22.960
Popis:	Aim. The aim of this study was to carry out passportization of cultivars, varieties and hybrids of perennial grass crops: Alopecurus protensis, Agropyron cristatum, Lolium perenne, Lolium miltiflorum, Festuca protensis, Festuca arundinasea, hybrids Festulolium. Methods. The perennial grasses selection was carried out by embryo culture according to methodical recommendations. For the molecular-genetic passportization of perennial grasses varieties the polymorphous primers have been selected – 9 RAPD primers and 15 ISSR primers. Results. Multilocus DNA labeling have allowed to differentiate all the genotypes being studied, to develop and to create the unique profiles for each of them and to calculate the genetic distance of congeniality/distances. On the base of RAPD/ISSR, SCoT spectra the 14 molecular-genetic passports of perennial grass cops have been obtained for the first time. Keywords: grass crops, molecular-genetic passport, DNA, PCR, multipoint mapping RAPD/ISSR spectra.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f934a367f750f209d88e680f4137d6d7 https://doi.org/10.7124/feeo.v22.960 Zobrazit plný text záznamu