| Popis: |
Microbial communities associated with invertebrates had been considered as a new source of bioactive compounds. The soft coral associated bacteria in Mot island, Nha Trang bay were isolated, extracted and assessed for antagonistic activity against human and coral pathogens, the strongly active strains were identified by 16S rRNA analysis. The soft coral associated bacterium SCN10 had abcd antibacterial pattern which was named for inhibition towards Bacillus subtilis (pattern a), Escherichia coli (pattern b), Salmonella typhimurium (pattern c) and Serratia marcescens (pattern d). It was the nearest strain to the well-known antibiotic producer Bacillus amyloliquefaciens with 99% sequence similarity. Whereas strain SCL19 had abde pattern which means inhibition of the growth of B. subtilis, E. coli, S. marcescens and Vibrio parahaemolyticus (pattern e). This strain SCL19 affiliated with Bacillus sp. strain A-3-23B with 99.8% identity. In addition to antimicrobial activity to the aforementioned tested bacteria, the isolate SCX15 also inhibited Vibrio alginolyticus (pattern f) and Candida albicans (pattern g), so this isolate possessed abcdefg antimicrobial pattern. The coral associated isolate SCX15 was identified as Bacillus velezensis with 99% sequence similarity. Among the 78 screened strains, 25 isolates possessed antibacterial activity against at least one of seven tested microorganisms and exhibited 12 different types of antimicrobial activities, suggesting that they can produce many different natural substances with antibacterial activity. |
