Direct Drug Determination by Selective Sample Enrichment on an Imprinted Polymer

Autor:	Boerje Sellergren
Rok vydání:	1994
Předmět:	chemistry.chemical_classification chemistry.chemical_compound Analyte Molecular recognition Chromatography Affinity chromatography chemistry Desorption Polymer Enrichment factor High-performance liquid chromatography Benzamidine Analytical Chemistry
Zdroj:	Analytical Chemistry. 66:1578-1582
ISSN:	1520-6882 0003-2700
Popis:	A new approach to sample enrichment and analyte determination is reported. An imprinted dispersion polymer capable of molecular recognition of pentamidine (PAM), a drug used for the treatment of AIDS-related pneumonia, was used in solid-phase extraction in order to selectively retain PAM from a dilute solution. At a physiological concentration (30 nM) this gave an enrichment factor of 54 using a PAM-selective polymer whereas the enrichment factor on a benzamidine- (BAM-) imprinted reference polymer was only 14. The high selectivity of the polymer allowed the drug to be detected directly in the desorption step, thus eliminating the need for a successive chromatographic analysis. In this way, PAM could be enriched and directly analyzed when present in low concentration in a urine sample
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f91bd13970647a555b1d92fcf1fd62db https://doi.org/10.1021/ac00081a036 Zobrazit plný text záznamu