Molecular Study of Enterotoxins Genes of Staphylococcus aureus Isolated from Patients in Intensive Care Units
| Autor: | Maysaa El Sayed Zaki, Mohamed Anies Rizk, Asmaa Osama Bakr |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
General Immunology and Microbiology
business.industry Enterotoxin 030501 epidemiology medicine.disease_cause Microbiology 03 medical and health sciences Enterotoxin gene 0302 clinical medicine Staphylococcus aureus Intensive care Multiplex polymerase chain reaction Medicine 030212 general & internal medicine 0305 other medical science business Gene |
| Zdroj: | The Open Microbiology Journal. 12:381-389 |
| ISSN: | 1874-2858 |
| DOI: | 10.2174/1874285801812010381 |
| Popis: | Introduction:Staphylococcus aureusis responsible for healthcare-associated sepsis. Various virulence genes may be responsible for the severity of the outcome of this infection.Aims & objectives:Therefore, the aim of the present study was to investigate the prevalence of enterotoxin genes inS .aureusassociated with HCA sepsis affecting adult's patients in Intensive Care Units (ICU).Materials & Methods:The study is a cross-sectional observational study included 432 patients admitted to intensive care units with Healthcare-Associated Infections (HCAIs). The patients were subjected to blood culture and identification of the isolated bacteria according to the standard microbiological method. Isolates identified asS. aureuswere subjected to molecular studies for the detection of meca gene and enterotoxins genes by multiplex Polymerase Chain Reaction (PCR).Results & Discussion:Blood culture was positive in 170 of the patients (39.3%). 47 of the blood culture yieldS. aureu.Regarding studies of virulence genes in isolatedS. aureus, the most frequent genes were seg (61.7%), mecA (46.8%) and sea (42.6%). The less frequent genes were tst and eta 6.4% for each. Analysis of demographic, clinical and virulence genes of isolatedS. aureusto HAIs outcome reveals that the virulence genes namely mec, sei and tst ofS. aureuswere the only significant factors associated with death The distribution of enterotoxigenic genes among MRSA and MSSA shows a significant association between seg and sei genes in MRSA isolates.Conclusion:The present study highlights the prevalence of enterotoxin genes in isolated S.aureus from patients with hospital-acquired infections in intensive care units in a tertiary hospital. There was high prevalence as about two-thirds of the isolates harbor seg and sea genes in addition to methicillin resistance gene mecA. The fatal outcome of sepsis in those patients was associated with the presence of enterotoxin genes sei and tst. There is a need for screening patients admitted to intensive care units forS. aureusharboring these genes for proper management and application of isolation and contact guidelines of infection control |
| Databáze: | OpenAIRE |
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