Automation of tacrolimus measurement on volumetric absorptive microsampling devices by tandem mass spectrometry

Autor:	Rachel S Carling, Catharine John, Erin C Emmett, Donna Austin, Colin Hastie, Camilla Liscio
Rok vydání:	2022
Předmět:	Medical Laboratory Technology Clinical Biochemistry General Medicine General Pharmacology Toxicology and Pharmaceutics Analytical Chemistry
Zdroj:	Bioanalysis. 14:1487-1496
ISSN:	1757-6199 1757-6180
DOI:	10.4155/bio-2022-0202
Popis:	Aims: An automated method for the measurement of blood tacrolimus on volumetric absorptive microsampling (VAMS) devices was developed. Materials & methods: VAMS devices prepared by the automated method were compared with those prepared by the existing manual method (n = 284; mean concentration: 8.0 μg/l; range: 0.6–18.1). Results: The performance of both methods was comparable. Passing–Bablok regression demonstrated an acceptable correlation (y = -0.449 + 1.06x). Bland-Altman analysis demonstrated acceptable agreement (mean bias: -0.007 μg/l; standard deviation: 1.536). Automation reduced operator touch time by 40 min (48-sample batch). Conclusion: Automated preparation of VAMS devices reduced touch time and improved process consistency, facilitating high-throughput testing and transformation of existing laboratory workflows. Automation did not improve precision for VAMS devices but did so for liquid blood samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f8fb2c34d073136e04fd9e67aeece03d https://doi.org/10.4155/bio-2022-0202 Zobrazit plný text záznamu