Determination of pholcodine in syrups and human plasma using the chemiluminescence system of tris(1,10 phenanthroline)ruthenium(II) and acidic Ce(IV)
| Autor: | Mohammad Reza Jamali, Nastaran Jafari Delouei, Ali Mokhtari |
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| Rok vydání: | 2016 |
| Předmět: |
Tris
Pholcodine Detection limit Phenanthroline 010401 analytical chemistry Inorganic chemistry Biophysics chemistry.chemical_element 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Dosage form 0104 chemical sciences law.invention Ruthenium chemistry.chemical_compound chemistry Chemistry (miscellaneous) law medicine 0210 nano-technology Derivative (chemistry) Chemiluminescence medicine.drug |
| Zdroj: | Luminescence. 32:387-393 |
| ISSN: | 1522-7235 |
| Popis: | Pholcodine is an opiate derivative drug which is widely used in pediatric medicine. In this study, a chemiluminescence (CL) method is described that determines pholcodine in human plasma and syrup samples. This method is based on the fact that pholcodine can greatly enhance the weak CL emission of reaction between tris(1,10 phenanthroline)ruthenium(II), Ru(phen)32+ , and acidic Ce(IV). The CL mechanism is described in detail using UV-vis light, fluorescence and CL spectra. Effects of chemical variables were investigated and under optimum conditions, CL intensity was proportional to the pholcodine concentration over the range 4.0 × 10-8 to 8.0 × 10-6 mol L-1 . The limit of detection (LOD) (S/N = 3) was 2.5 × 10-8 mol L-1 . Percent of relative standard deviations (%RSD) for 3.0 × 10-7 and 3.0 × 10-6 mol L-1 of pholcodine was 2.9 and 4.0%, respectively. Effects of common ingredients were investigated and the method was applied successfully to the determination of pholcodine in syrup samples and human plasma. |
| Databáze: | OpenAIRE |
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