| Autor: |
O. Abas Mazni, M. M. Bukar, H. Wahid, Hadi Hajarian, Y. Rosnina, M. Daliri, Mojtaba Dashtizad, Nurhusien Yimer, Tooba Mirzapour, M. I. Iswadi |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Journal of Animal and Veterinary Advances. 10:2541-2545 |
| ISSN: |
1680-5593 |
| DOI: |
10.3923/javaa.2011.2541.2545 |
| Popis: |
The aim of this study was to evaluate the effectiveness of different cryodevices (Open Pulled Straw (OPS), Electron Microscopy Grid (EMG) and cryotop for vitrification of immature bovine oocytes. Polar body, MII stage, survivability and subsequent developmental rates were compared. Only oocytes with 4-5 layers of cumulus cells were used. Oocytes were equilibrated in the first vitrification solution (VS1; HS+10% DMSO+10% Ethylene Glycol (EG)) for 30-45 sec and then in the second vitrification solution (VS2; 20% DMSO+20% EG+0.5 M Sucrose) for 25 sec. Within 30 sec they were mounted on one of the cryodevices and directly plunged into Liquid Nitrogen (LN2) for 10 days. Immature oocytes vitrified using cryotop represented higher rate of polar body extrusion and nuclear maturity (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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