Fertility of bull semen packaged in .25- and .5-milliliter french straws2
| Autor: | B.M. Alexander, D D Hancock, R.G. Sasser, P. L. Senger, M S Johnson, C H Allen |
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| Rok vydání: | 1995 |
| Předmět: | |
| Zdroj: | Journal of Animal Science. 73:1914-1919 |
| ISSN: | 1525-3163 0021-8812 |
| DOI: | 10.2527/1995.7371914x |
| Popis: | The fertility of bull semen packaged in .25- and .5-mL french straws was compared. One ejaculate from each of five Holstein bulls was split, extended to 10 x 10(6) spermatozoa/inseminate dose in whole homogenized milk, packaged in .25- and .5-mL french straws, frozen in liquid nitrogen (LN) vapor, and stored in LN. Semen was thawed at 37 degrees C for 30 s. Synchronized heifers (n = 1,360) were inseminated (during a 12-mo period) with semen packaged in either a .25- or .5-mL french straw. Blood was collected on the day of insemination and the serum was assayed for progesterone. Heifers with blood progesterone levels of > 1 ng/mL were eliminated from the data. Blood was collected at 30 to 45 d after insemination and the serum was assayed for the presence of bovine pregnancy-specific protein B (bPSPB) by RIA to determine pregnancy. Conception was 63.6 and 62.0% (P = .55) for semen packaged in the .25- and .5-mL french straws, respectively. There was neither a bull x packaging unit interaction (P = .49) nor a day of insemination x packaging unit interaction (P = .87). Conception among bulls ranged from 57.1 to 68.0% (P = .19). No evidence was found that meteorological factors influenced conception. Under the conditions of this experiment, semen packaged in the .25- and .5-mL french straw had similar fertility. |
| Databáze: | OpenAIRE |
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