Luciferase Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus
| Autor: | Vanina Dengler, Nadine McCallum |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology medicine.drug_class Chemistry 030106 microbiology Antibiotics Cell biology.organism_classification medicine.disease_cause Molecular biology Fusion gene Cell wall 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Staphylococcus aureus medicine Cell envelope Psychological repression Bacteria |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493936748 |
| DOI: | 10.1007/978-1-4939-3676-2_11 |
| Popis: | Luciferase reporter gene fusions provide an extremely rapid and sensitive tool for measuring the induction or repression of stress responses in bacteria. Staphylococcus aureus activates the expression of a cell wall stress stimulon (CWSS) in response to the inhibition or disruption of cell wall synthesis. The highly sensitive promoter-reporter gene fusion construct psas016 p-luc+ can be used to quantify and compare any changes in CWSS expression levels and induction kinetics. Potential uses of this system include identifying and characterizing novel cell wall-targeting antibacterial agents, identifying genomic loci influencing cell envelope synthesis and detecting changes in CWSS expression that could be linked to decreased antibiotic susceptibility profiles in clinical isolates. |
| Databáze: | OpenAIRE |
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