Protein and Protease Sensing by Allosteric Derepression
| Autor: | Farid J. Ghadessy, Hui Chin Goh, Saurabh Nirantar |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Protease biology Chromogenic medicine.medical_treatment Allosteric regulation Peptide Fluorescence Protein–protein interaction 03 medical and health sciences 030104 developmental biology Biochemistry chemistry Allosteric enzyme medicine biology.protein Derepression |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493969388 |
| DOI: | 10.1007/978-1-4939-6940-1_11 |
| Popis: | Peptide motifs are crucial mediators of protein-protein interactions as well as sites of specific protease activity. The detection and characterization of these events is therefore indispensable for a detailed understanding of cellular regulation. Here, we present versatile and modular sensors that allow the user to detect protease activity and protein-peptide interactions, as well as to screen for inhibitors using chromogenic, fluorescent, or luminescent output. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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